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Updated: Feb 2, 2026

Field Experiments of Pollination Ecology: The Case of Lycoris sanguinea var. sanguinea
Published on: November 25, 2016
Development of 17 novel microsatellite markers for Lycoris aurea and L. radiata (Amaryllidaceae) using
I-Ju Chen1, Chou-Tou Shii1, Tsu-Liang Chang1
1Department of Horticulture and Landscape Architecture National Taiwan University No. 1, Sec. 4, Roosevelt Road Taipei 10617 Taiwan.
Premise Of The Study:
Lycoris is an ornamental and medicinal plant. We developed microsatellite markers for L. aurea and L. radiata simultaneously by using a hybrid between these two species.
Methods And Results:
Ion Torrent next-generation sequencing produced 1,784,504 reads. Testing 64 primer sets allowed for the development of 17 novel microsatellite markers: 16 for L. aurea, 10 for L. radiata, and nine common markers. Lycoris aurea had one to 12 alleles per locus and observed and expected heterozygosity levels of 0-0.923 and 0.038-0.809, respectively. Lycoris radiata had three to 12 alleles per locus and observed and expected heterozygosity levels of 0-0.909 and 0.127-0.797, respectively. Ten markers were cross-amplified for L. sprengeri.
Conclusions:
Hybrid sequencing can facilitate the cost-effective development of molecular markers for parental species. The markers developed here are useful for studying Lycoris population structure.
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