Related Experiment Video
Updated: Feb 2, 2026

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
Published on: March 3, 2011
DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA)
Alessandro Galbiati1, Fabrizio d'Adda di Fagagna2,3
1Oncology IMED, AstraZeneca UK Ltd, Cambridge, UK. alessandro.galbiati@astrazeneca.com.
Abstract:
Cells have evolved DNA repair mechanisms to maintain their genetic information unaltered and a DNA damage response pathway that coordinates DNA repair with several cellular events. Despite a clear role for DNA damage in the form of DNA double-strand breaks (DSBs) in several cellular processes, the most commonly used methods to detect DNA lesions are indirect, and rely on antibody-based recognition of DNA damage-associated factors, leaving several important questions unanswered. Differently, here we describe DNA damage In situ ligation followed by Proximity Ligation Assay (DI-PLA), that allows sensitive detection of physical DSBs in fixed cells, through direct labeling of the DSBs with biotinylated oligonucleotides, and subsequent signal amplification by PLA between biotin and a partner protein in the proximity of the DNA break.
Related Concept Videos
DNA Damage Can Stall the Cell Cycle
DNA Damage can Stall the Cell Cycle
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
Overview of DNA Repair
Chemically...
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Factors Influencing Attraction I: Proximity

