Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

4.4K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.4K
Responses to Salt Stress02:02

Responses to Salt Stress

14.6K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.6K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

14.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.8K
Stress01:20

Stress

8.7K
When a force is applied on a body, it undergoes deformation. In order to restore the body to its original shape and/or size, an opposite or restoring force is generated within the body. This restoring force is equal to the magnitude of the applied force, but acts in the opposite direction. The amount of this restoring force developed per unit area of the body is called stress. Stress is a tensor quantity and has the SI unit pascal. Stress can be separated into four broad categories depending...
8.7K
General State of Stress01:21

General State of Stress

660
The general state of stress within a material can be accurately depicted using a stress tensor. This tensor encapsulates the internal forces distributed within a material subjected to external forces or deformations.
Specifically, consider a tetrahedral element where one face, labeled XYZ, is perpendicular to the line OA, and the remaining faces align with the coordinate axes with point O as the origin. At any point, such as point O, the stress tensor can be used to determine the stress...
660
Stress Prevention and Stress Management Techniques II01:23

Stress Prevention and Stress Management Techniques II

530
Personality types, particularly Type A and Type B, significantly influence how individuals respond to stress. These personality distinctions are marked by varying levels of ambition, competitiveness, and coping styles, all of which shape an individual's resilience to stressors.
Type A Personality: Driven and Easily Stressed
Individuals with Type A personalities are often highly competitive and ambitious and operate with a strong sense of urgency. Commonly labeled as...
530

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous auto-transplant and CD19 CAR T-cell 2nd line therapy followed by CD20 × CD3 bispecific antibody for refractory Burkitt lymphoma, a case report.

Leukemia & lymphoma·2025
Same author

Erratum: Cell-based immune anticipation of the omicron variant in SARS-CoV-2 triple-vaccinated cancer patients.

iScience·2025
Same author

Cell-based immune anticipation of the omicron variant in SARS-CoV-2 triple-vaccinated cancer patients.

iScience·2025
Same author

lncRNA IGFL2-AS1 mediates NSCLC chemoresistance via YBX1-induced HSPA1A/RAP1 activation.

Cellular & molecular biology letters·2025
Same author

Chronic Stress Stimulates Protumor Macrophage Polarization to Propel Lung Cancer Progression.

Cancer research·2025
Same author

Senescence-associated lineage-aberrant plasticity evokes T-cell-mediated tumor control.

Nature communications·2025

Related Experiment Video

Updated: Feb 2, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.1K

Genotoxic Stress-Induced Senescence.

Dorothy N Y Fan1,2,3, Clemens A Schmitt4,5,6

  • 1Department of Hematology, Oncology and Tumor Immunology, Molekulares Krebsforschungszentrum - MKFZ, Charité - University Medical Center, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2018
PubMed
Summary

Cellular senescence, a cell-cycle exit program, is crucial for limiting damaged cell propagation. New methods using fluorescent senescence-associated β-galactosidase (fSA-β-gal) allow tracking of viable senescent cells over time.

Keywords:
5-Ethynyl-2′-deoxyuridine (EdU)BiomarkerCell fateFluorescent SA-β-gal (fSA-β-gal)Genotoxic stressH3K9me3Ki67SenescenceSenescence-associated β-galactosidase (SA-β-gal)Single cellTherapy-induced senescence (TIS)

More Related Videos

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.6K
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
09:14

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Published on: October 26, 2017

10.1K

Related Experiment Videos

Last Updated: Feb 2, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.1K
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.6K
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
09:14

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

Published on: October 26, 2017

10.1K

Area of Science:

  • Cellular Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Genomic integrity is threatened by DNA-damaging stress from oncogenes or genotoxic treatments.
  • Cellular failsafe programs, including apoptosis and senescence, prevent propagation of damaged cells.
  • Cellular senescence is a persistent cell-cycle exit program with significant roles in tissue and tumor microenvironments.

Purpose of the Study:

  • To establish a clinically relevant model for studying therapy-induced senescence (TIS).
  • To develop novel methods for detecting viable senescent cells.
  • To enable real-time tracking of single senescent cells over time.

Main Methods:

  • Utilized apoptosis-compromised, Bcl2-expressing Eμ-myc transgenic mouse lymphomas as a model system.
  • Developed novel detection methods combining fluorescent senescence-associated β-galactosidase (fSA-β-gal) assay.
  • Integrated fSA-β-gal with immunofluorescence staining for H3K9me3 or Ki67, and 5-ethynyl-2'-deoxyuridine (EdU) incorporation for DNA replication analysis.

Main Results:

  • Established a robust model for studying therapy-induced senescence.
  • Demonstrated novel methods for detecting viable senescent cells using fSA-β-gal.
  • Showcased the capability of fSA-β-gal to track single-cell fate in senescent populations over time.

Conclusions:

  • Cellular senescence is a critical failsafe mechanism distinct from apoptosis.
  • Novel fSA-β-gal based assays provide a powerful tool for studying senescent cells.
  • These methods allow for dynamic monitoring of senescent cell behavior, advancing our understanding of their roles in disease and therapy.