Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What are Carbohydrates?01:44

What are Carbohydrates?

198.7K
Overview
198.7K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

90.6K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
90.6K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

10.7K
10.7K
Carbohydrate Digestion00:57

Carbohydrate Digestion

122.5K
Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. Carbohydrate digestion starts in the mouth during mastication, or chewing. The masticated carbohydrates remain intact in the stomach. Digestion resumes in the duodenum of the small intestine, where pancreatic alpha-amylase and brush border enzymes of the microvilli convert complex carbohydrates to monosaccharides. Finally, the monosaccharides...
122.5K
Carbohydrate Metabolism01:36

Carbohydrate Metabolism

14.2K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
14.2K
Membrane Carbohydrates01:30

Membrane Carbohydrates

7.4K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gel-Based NMR Method for Observing Submicrosecond Protein Dynamics at Atomic Resolution.

The journal of physical chemistry letters·2026
Same author

DEEP Phaser: A Deep Learning Tandem Vision Transformer for Fully Automated NMR Phase Correction.

The journal of physical chemistry letters·2026
Same author

Detection of intramolecular protein dynamics on nanosecond-to-microsecond timescales by nanoparticle-assisted NMR spin relaxation (NASR).

Nature protocols·2025
Same author

COLMAR1d2d: Synergistic Combination of 1D with 2D NMR for Enhanced High-Throughput Identification and Quantification of Metabolites in Complex Mixtures.

Analytical chemistry·2025
Same author

Dynamics of Globular Proteins when Interacting with Zwitterionic Silica Nanoparticles by Nuclear Magnetic Resonance Spin Relaxation.

Journal of the American Chemical Society·2025
Same author

Coil-Library-Derived Amino-Acid-Specific Side-Chain χ<sub>1</sub> Dihedral Angle Potentials for AMBER-Type Protein Force Field.

Journal of chemical theory and computation·2024

Related Experiment Video

Updated: Feb 2, 2026

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.6K

Carbohydrate Background Removal in Metabolomics Samples.

Jiaqi Yuan1, Bo Zhang1, Cheng Wang1

  • 1Department of Chemistry and Biochemistry , The Ohio State University , Columbus , Ohio 43210 , United States.

Analytical Chemistry
|November 27, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a simple chemical method using sodium periodate to remove carbohydrate signals from NMR metabolomics data. This simplifies spectra, enabling clearer identification of other important metabolites in biological samples.

More Related Videos

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.8K
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

6.3K

Related Experiment Videos

Last Updated: Feb 2, 2026

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.6K
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.8K
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

6.3K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Metabolomics

Background:

  • NMR-based metabolomics is crucial for studying biological systems.
  • Metabolite identification and quantification are key but challenged by NMR spectral overlap, particularly for carbohydrates.
  • The 3.2-4.5 ppm 1H NMR region is often obscured by carbohydrate signals.

Purpose of the Study:

  • To develop an effective method for removing carbohydrate signals from complex metabolomics samples.
  • To simplify NMR spectra for improved metabolite identification and quantification.
  • To enhance the detection of non-carbohydrate metabolites in biological samples.

Main Methods:

  • Utilized oxidation with sodium periodate (NaIO4) to remove carbohydrate signals.
  • Incorporated an optional step using hydrazide beads to remove reaction products.
  • Applied the method to both model mixtures and biological samples (urine, serum).

Main Results:

  • Demonstrated substantial simplification of 1D and 2D NMR spectra after carbohydrate removal.
  • Confirmed that non-carbohydrate peaks remained largely unaffected by the treatment.
  • Identified a significant number of previously obscured non-carbohydrate metabolites.

Conclusions:

  • The sodium periodate oxidation method effectively removes carbohydrate signals in NMR metabolomics.
  • This technique enhances the detection and identification of non-carbohydrate metabolites.
  • The protocol is simple, suitable for high-throughput analysis, and applicable to diverse biological samples.