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Updated: Feb 2, 2026

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
Doxorubicin Induces ER Calcium Release via Src in Rat Ovarian Follicles
Aziz Ur Rehman Aziz1, Chunyang Geng1, Wang Li1
1Liaoning IC Technology Key Laboratory, School of Biomedical Engineering, Dalian University of Technology, Dalian, Liaoning 116024, P. R. China.
Abstract:
Although doxorubicin has been widely used as an anticancer drug, it has dose-dependent toxic effects on ovarian follicle development and apoptosis, oocyte maturation, and hormone secretion. Ca2+ signaling also has vital roles in the same cellular functions of ovarian follicles, indicating a strong link with doxorubicin-induced ovarian toxicity. In the current project, doxorubicin-induced Ca2+ alternations in cultured rat ovarian follicles have been explored with the fluorescence resonance energy transfer technology. The results reveal that doxorubicin enhances the cytosolic Ca2+ level smoothly. Further experiments confirm that the endoplasmic reticulum (ER) calcium, but not the extracellular calcium influx, is the main source of intracellular calcium increase. Moreover, Src kinase activation could be the upstream of doxorubicin-induced ER calcium release. Therefore, this project demonstrates that doxorubicin increases the cytosolic Ca2+ mainly by releasing calcium from ER via Src kinase activation in ovarian follicles, which provides deeper understanding of doxorubicin-induced ovarian toxicity.
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