[Adaptation of a diazoreaction method for determining total and conjugated bilirubins on a parallel analyser]

A diazoreaction method of estimation of bilirubin has been adapted on a FP9 parallel analyzer. Biotrol's kit could be used only after modifications of the protocol. The critical study of this adaptation included trials of within run precision (CV 1.93 to 4.42%), total precision (CV 2.59 to 6.66%) as also evaluation of analytical range: the limit of linearity is 340 mumol/l. The evaluation of inaccuracy performed with patient specimens leads to establishment of follow-up norms and interpretation norms of allometry line. Our whole results are in conformity with the performance standards of the protocol for the validation of methods published by the Société Française de Biologie Clinique. Finally the described method is quick acting, reliable and very inexpensive.