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Updated: Feb 2, 2026
09:04
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Published on: September 25, 2019
8.8K
Modeling Human Digestive Diseases With CRISPR-Cas9-Modified Organoids
Masayuki Fujii1, Hans Clevers2, Toshiro Sato1
1Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan.
Gastroenterology
|November 27, 2018
Summary
Researchers are using organoids derived from human stem cells to model diseases and test drugs. CRISPR-Cas9 gene editing enhances these organoids for studying genetic disorders and cancer in the digestive system.
Area of Science:
- Gastroenterology
- Stem Cell Biology
- Gene Editing
Background:
- Stem cell niche insights enable cultivation of adult tissue stem cells into organoids.
- These organoids mimic healthy and diseased epithelial tissues.
- Patient-derived organoids serve as models for disease and drug testing.
Purpose of the Study:
- To review the derivation of organoids from human gastrointestinal tissues.
- To explore the application of CRISPR-Cas9 technology in studying these organoids.
- To discuss emerging technologies for understanding digestive system diseases.
Main Methods:
- Cultivation of adult stem cells into organoids.
- Genetic engineering of organoids using CRISPR-Cas9 technology.
- Review of organoid derivation and applications in gastrointestinal research.
Main Results:
- Organoids accurately model structural and phenotypic features of epithelial tissues.
- CRISPR-Cas9 enables genetic engineering for disease modeling, including monogenic diseases and cancer.
- Organoids provide a platform for drug screening and disease mechanism studies.
Conclusions:
- Gastrointestinal organoids are valuable tools for disease modeling and drug discovery.
- CRISPR-Cas9 technology significantly enhances the utility of organoids for genetic studies.
- Advancements in organoid technology and gene editing promise deeper insights into digestive diseases.
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