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Updated: Feb 2, 2026
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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
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p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
Luis G Rivera-Calderón1, Carlos E Fonseca-Alves2, Priscila E Kobayashi2
1Department of Veterinary Pathology, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, São Paulo, Brazil.
Research in Veterinary Science
|November 27, 2018
Summary
The mechanistic target of rapamycin (mTOR)/4E-binding protein 1 (4E-BP1)/eukaryotic initiation factor 4E (eIF4E) pathway is altered in canine prostate cancer (PC). Higher p-mTOR and eIF4E protein levels correlate with higher Gleason scores in canine PC.
Area of Science:
- Comparative oncology
- Molecular biology
- Cancer research
Background:
- The mTOR/4E-BP1/eIF4E pathway is crucial in neoplastic transformation and tumor growth.
- This pathway is altered in human prostate cancer (PC).
- Canine PC is a frequent cancer and a valuable model for comparative oncology.
Purpose of the Study:
- Investigate mTOR, 4E-BP1, and eIF4E gene and protein expression in canine PC.
- Evaluate correlations between expression and Gleason score (GS).
- Analyze expression in normal, PC, and metastatic tissues.
Main Methods:
- Quantitative PCR (qPCR) and immunohistochemistry on formalin-fixed paraffin-embedded (FFPE) tissues.
- Analysis of 35 samples: 13 normal prostate, 17 PC, 5 metastasis.
- Investigation of mTOR kinase domain gene mutations.
Main Results:
- Higher p-mTOR and eIF4E protein levels observed in canine PC with higher Gleason scores (≥ 8).
- Significant positive correlation found between p-mTOR and eIF4E protein expression.
- eIF4E overexpression noted in metastatic samples compared to normal tissues.
Conclusions:
- p-mTOR and eIF4E expression positively correlates with Gleason score in canine PC, mirroring human patterns.
- Further research needed to explore protein correlations with clinical findings and therapeutic potential in canine PC.
- The mTOR/4E-BP1/eIF4E pathway warrants deeper investigation as a therapeutic target in canine PC.
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