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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

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CircRNA ZNF609 functions as a competitive endogenous RNA to regulate FOXP4 expression by sponging miR-138-5p in renal

Yunhe Xiong1, Jiabin Zhang2, Chao Song1

  • 1Urology Department, Renmin Hospital of Wuhan University, Wuhan, China.

Journal of Cellular Physiology
|November 28, 2018
PubMed

Insights

Circular RNA ZNF609 (circ-ZNF609) is elevated in renal cell carcinoma and promotes cancer cell proliferation and invasion. This study reveals the circ-ZNF609/miR-138-5p/FOXP4 network

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in various disease pathologies.
  • Elevated circRNA ZNF609 (circ-ZNF609) expression is observed in renal cell carcinoma (RCC).
  • circ-ZNF609 is known to influence cell proliferation and invasion in other cell types.

Purpose of the Study:

  • To investigate the role of circ-ZNF609 in renal carcinoma cell proliferation and invasion.
  • To elucidate the regulatory network involving circ-ZNF609, microRNA-138-5p (miR-138-5p), and forkhead box P4 (FOXP4) in RCC.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure circ-ZNF609 expression.
  • Luciferase reporter and RNA immunoprecipitation assays to confirm molecular interactions.
  • Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine (EdU), and Matrigel assays to assess cell proliferation and invasion.

Main Results:

  • circ-ZNF609 expression is significantly upregulated in renal carcinoma cell lines compared to normal renal epithelial cells.
  • High expression of circ-ZNF609 enhances the proliferation and invasion capabilities of renal carcinoma cells.
  • The study confirmed direct interactions between miR-138-5p and both FOXP4 and circ-ZNF609.

Conclusions:

  • circ-ZNF609 acts as a promoter of proliferation and invasion in renal carcinoma.
  • The circ-ZNF609/miR-138-5p/FOXP4 regulatory axis plays a crucial role in the pathogenesis of renal carcinoma.
  • This regulatory network offers a novel perspective for understanding RCC development and potential therapeutic targets.
Keywords:
cell invasioncell proliferationcircRNAcompeting endogenous RNAskidney cancer

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