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Updated: Feb 2, 2026

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
Published on: August 4, 2022
MFF-dependent mitochondrial fission regulates presynaptic release and axon branching by limiting axonal mitochondria
Tommy L Lewis1,2,3, Seok-Kyu Kwon1,2,4, Annie Lee1,2
1Department of Neuroscience, Columbia University, New York, NY, 10032, USA.
Abstract:
Neurons display extreme degrees of polarization, including compartment-specific organelle morphology. In cortical, long-range projecting, pyramidal neurons (PNs), dendritic mitochondria are long and tubular whereas axonal mitochondria display uniformly short length. Here we explored the functional significance of maintaining small mitochondria for axonal development in vitro and in vivo. We report that the Drp1 'receptor' Mitochondrial fission factor (MFF) is required for determining the size of mitochondria entering the axon and then for maintenance of their size along the distal portions of the axon without affecting their trafficking properties, presynaptic capture, membrane potential or ability to generate ATP. Strikingly, this increase in presynaptic mitochondrial size upon MFF downregulation augments their capacity for Ca2+ ([Ca2+]m) uptake during neurotransmission, leading to reduced presynaptic [Ca2+]c accumulation, decreased presynaptic release and terminal axon branching. Our results uncover a novel mechanism controlling neurotransmitter release and axon branching through fission-dependent regulation of presynaptic mitochondrial size.
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