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Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
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Matrix metalloproteinases in ureteropelvic junction obstruction
A Pavlaki1, N Printza1, E Farmaki1
1First Department of Pediatrics, Hippokratio Hospital, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Hippokratia
|November 28, 2018
Summary
Matrix metalloproteinase-9 (MMP9) levels were significantly lower in infants with ureteropelvic junction obstruction (UPJO). Further research is needed to confirm MMPs as reliable biomarkers for UPJO.
Area of Science:
- Pediatric Urology
- Biomarker Discovery
- Renal Physiology
Background:
- Ureteropelvic junction obstruction (UPJO) management is debated.
- Matrix metalloproteinases (MMPs) role in UPJO is unclear.
- Investigating MMPs as potential UPJO biomarkers is crucial.
Purpose of the Study:
- To quantify serum MMP2 and MMP9 levels in infants with UPJO.
- To determine if MMP levels can serve as biomarkers for surgical obstruction in UPJO.
Main Methods:
- Serum samples from 17 infants with UPJO (median age 1.5 months) and healthy controls were analyzed.
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify MMP2 and MMP9.
Main Results:
- MMP9 levels were significantly decreased in infants with UPJO compared to controls (p=0.037).
- MMP2 levels were higher in UPJO infants, but not statistically significant (p=0.206).
Conclusions:
- Infants with obstructive hydronephrosis showed reduced MMP9 concentrations.
- Larger studies and simultaneous urine sample analysis are recommended to validate MMPs as UPJO biomarkers.
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