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Updated: Feb 2, 2026

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation
Published on: June 16, 2023
Vacuum-assisted closure (VAC®) systems and microbiological isolation of infected wounds
Valerio Cozza1,2, Gilda Pepe1, Marco Cintoni1
11Emergency Surgery Department, Università Cattolica del Sacro Cuore, Fondazione Policlinico A. Gemelli IRCCS, Rome, Italy.
Background:
Negative pressure wound therapy is now largely used to treat infected wounds. The prevention and reduction of healthcare-associated infections is a high priority for any Department of Health and great efforts are spent to improve infection control systems. It is assumed that vacuum-assisted closure (VAC®) dressings should be watertight and that all the secretions are gathered in a single container but there is no consistent data on air leakage and possible dispersion of bacteria from the machine.
Methods:
We have conducted a prospective experimental study on 10 patients with diagnosis of wound infection to verify whether the filtration process is microbiologically efficient. We compared the bacteria population present in the wound to the one present in the air discharged by the VAC® machine.
Results:
This study shows that the contamination of the VAC® machine is considerably lower than the environment or wound contamination.
Conclusions:
Negative pressure wound therapy system does not represent a risk factor for healthcare-associated infections.
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