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Updated: Feb 2, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Autonomic Behaviors in Lipase-Active Oil Droplets
Lei Wang1, Youping Lin1, Yuting Zhou1
1MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry & Chemical Engineering, Harbin Institute of Technology (HIT), Harbin, 150001, China.
Abstract:
Developing self-fueled micro-reactor droplets with programmable autonomic behaviors provides a step towards smart liquid dispersions comprising motile microscale objects. Herein, we prepare aqueous suspensions of lipase-coated oil globules comprising a mixture of a triglyceride substrate (tributyrin, 1,2,3-tributylglycerol) and a low-density oil (polydimethylsiloxane, PDMS) and describe a range of active behaviors based on controlled enzyme-mediated consumption of individual droplets under non-equilibrium conditions. Encapsulation of the lipase-coated lipid/PDMS droplets into a model protocell as energy-rich sub-compartments is demonstrated as an internalized mechanism for activating protocell buoyancy. Taken together, our results highlight opportunities for the regulation of autonomic behavior in enzyme-powered oil droplets and provide a new platform for increasing the functionality and energization of synthetic protocells.
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