Related Experiment Video
Updated: Feb 2, 2026
09:00
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
Published on: April 18, 2025
1.4K
Proteomic approaches for cancer epigenetics research
Dylan M Marchione1, Benjamin A Garcia1, John Wojcik2
1a Epigenetics Institute, Department of Biochemistry and Biophysics, Perelman School of Medicine , University of Pennsylvania , Philadelphia , PA , USA.
Expert Review of Proteomics
|November 29, 2018
Summary
Proteomics reveals how epigenetic changes, like histone modifications and protein complex alterations, drive cancer. This research identifies new therapeutic targets for cancer epigenetics and treatment.
Area of Science:
- Cancer Epigenetics
- Proteomics
- Mass Spectrometry
Background:
- Epigenetic dysregulation is a hallmark of human cancers.
- Cancer cells exhibit altered chromatin landscapes, including abnormal histone post-translational modifications (PTMs) and aberrant protein complex assembly.
- Mass spectrometry-based proteomics is a key technology for studying these epigenetic alterations.
Purpose of the Study:
- To review and categorize proteomic approaches in cancer epigenetics research.
- To highlight the role of proteomics in understanding histone PTMs and protein-protein interactions in cancer.
- To discuss current limitations and future innovations in the field.
Main Methods:
- Literature review of 'modification-centric' and 'complex-centric' proteomic analyses.
- Analysis of proteomic studies on oncohistones.
- Discussion of emerging proteomic techniques.
Main Results:
- 'Modification-centric' analyses link histone PTMs to cancer biology and therapeutic vulnerabilities.
- 'Complex-centric' analyses uncover oncogenic mechanisms and potential therapeutic targets, especially in MLL-associated leukemia.
- Proteomics has supported studies on oncohistone-mediated tumorigenesis.
Conclusions:
- Proteomic approaches are crucial for dissecting cancer epigenetics.
- Future applications include advanced histone PTM analysis, chromatin reader profiling, and locus-specific protein identification.
- Proteomics will continue to be a powerful tool for cancer biology research.
Related Concept Videos
01:46Epigenetic Regulation
33.8K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.8K
01:37Epigenetic Regulation
3.9K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.9K
01:33Proteomics
9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K
01:20Frustration and Conflict: Approach-Approach, Approach-Avoidance
532
Frustration occurs when people are obstructed or prevented from achieving a desired goal or fulfilling a perceived need. For example, when someone's input is ignored in a discussion, it can lead to feelings of frustration. Conflict, however, arises from opposing interests, goals, or actions. Conflicts can take various forms based on the nature of these opposing desires or goals.
One common type of conflict is the Approach–Approach Conflict. In this case, a person faces two desirable...
One common type of conflict is the Approach–Approach Conflict. In this case, a person faces two desirable...
532
01:20Actuarial Approach
308
The actuarial approach, a statistical method originally developed for life insurance risk assessment, is widely used to calculate survival rates in clinical and population studies. This method accounts for participants lost to follow-up or those who die from causes unrelated to the study, ensuring a more accurate representation of survival probabilities.
Consider the example of a high-risk surgical procedure with significant early-stage mortality. A two-year clinical study is conducted,...
Consider the example of a high-risk surgical procedure with significant early-stage mortality. A two-year clinical study is conducted,...
308
01:30Information Processing Approach
569
The information-processing theory of cognitive development centers on fundamental mental processes, including attention, memory, and problem-solving skills. Researchers in this field examine how cognitive abilities, such as working memory, evolve and influence children's overall development. Studies indicate that children with stronger working memory tend to excel in reading comprehension, math, and problem-solving compared to peers with less efficient memory skills. Low working memory is...
569