Concentration cell-based potentiometric analysis for point-of-care testing with minimum background

One of the most critical problems of point-of-care testing is how to reduce the interference of background, especially under resource-limited conditions when sample pretreatment is not available. In this work we report a potentiometric method for point-of-care testing with minimum background. The method is based on the principles of a concentration cell which is a type of galvanic cells. It is an electrochemical cell having two carbon electrodes. The potential of each electrode is determined by ratio of a redox couple (i.e. Fe(CN)₆^4-/3-) on the electrode surface. On one electrode, the adsorbed enzyme catalyzes the oxidation of analyte by Fe(CN)₆^3- which produces Fe(CN)₆^4-. The shift of the potential was because of the analyte as well as the background. In the other channel, no enzyme was present so that the shift of the potential, if any, is owing to the background. By measuring the potential difference between the two electrodes (i.e. voltage of the concentration cell), analyte can be quantitatively determined with most of the background eliminated. As the proof-of-concept analyte, blood glucose is quantitatively detected using a voltammeter with acceptable selectivity and accuracy. Noble metal electrodes that are indispensable for conventional electrochemical sensing are not required. All these features simplify the fabrication procedure and reduce the cost for the detection. Therefore, we believe it is promising for electrochemical point-of-care testing.