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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
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Tutorial: Volumetric absorptive microsampling (VAMS).

Michele Protti1, Roberto Mandrioli2, Laura Mercolini1

  • 1Pharmaco-Toxicological Analysis Laboratory (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Bologna, Italy.

Analytica Chimica Acta
|November 29, 2018
PubMed
Summary
This summary is machine-generated.

Volumetric absorptive microsampling (VAMS) offers advantages over dried blood spot (DBS) techniques, including improved accuracy and automation. This tutorial explores VAMS procedures and results, aiding scientists in microsampling advancements.

Keywords:
BioanalysisMicrosamplingMiniaturisationSample treatmentVAMS

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Area of Science:

  • Analytical Chemistry
  • Bioanalysis
  • Biotechnology

Background:

  • Dried blood spot (DBS) sampling has been used for decades.
  • Microsampling techniques are crucial for bioanalytical applications.
  • Volumetric absorptive microsampling (VAMS) is an emerging alternative.

Purpose of the Study:

  • To provide a tutorial on the Volumetric Absorptive Microsampling (VAMS) technique.
  • To compare VAMS with traditional Dried Blood Spot (DBS) methods.
  • To highlight the advantages and potential of VAMS in bioanalysis.

Main Methods:

  • Description of VAMS procedures and analytical workflows.
  • Discussion of VAMS advantages: volume accuracy, hematocrit independence, pre-treatment, and automation.
  • Review of published results and methodologies using VAMS.

Main Results:

  • VAMS demonstrates significant advantages over DBS.
  • Key benefits include enhanced sampling volume accuracy and reduced hematocrit dependence.
  • VAMS offers improved pre-treatment options and automation potential.

Conclusions:

  • VAMS is a promising and viable alternative to DBS and other dried microsampling techniques.
  • Further research is needed to fully explore VAMS capabilities.
  • VAMS can help address existing challenges in biological sample collection and analysis.