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Related Experiment Videos

Transactivation by the adenovirus E1A gene.

J R Nevins1, P Raychaudhuri, A S Yee

  • 1Howard Hughes Medical Institute, Duke University Medical Center, Department of Microbiology-Immunology, Durham, NC 27710.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|June 1, 1988
PubMed
Summary
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Adenovirus E1A protein activates viral gene transcription indirectly by increasing cellular transcription factors like E2F and E4F. This coordinate control involves modifying pre-existing factors, not requiring new protein synthesis.

Area of Science:

  • Molecular Biology
  • Virology
  • Gene Regulation

Background:

  • The adenovirus E1A gene product (289aa) is crucial for activating early viral and cellular gene transcription.
  • E1A protein functions indirectly, without direct DNA binding, by modulating cellular transcription factors.

Purpose of the Study:

  • To investigate the mechanism by which the adenovirus E1A gene product mediates transcriptional activation.
  • To identify and characterize cellular transcription factors influenced by E1A.

Main Methods:

  • Analysis of transcription factors in nuclear extracts.
  • Purification of specific transcription factors (E2F and E4F).
  • In vivo assays to assess promoter complex formation.

Main Results:

Related Experiment Videos

  • E1A increases the concentration and DNA binding activity of E2F (specific for the E2 promoter) and E4F (specific for the E4 promoter).
  • E1A-mediated increase in factor activity does not require protein synthesis, suggesting modification of pre-existing factors.
  • E2F (54kDa) and E4F (50kDa) were purified to homogeneity.

Conclusions:

  • Adenovirus E1A achieves coordinate transcriptional control by activating multiple promoter-specific cellular factors.
  • The E1A mechanism involves post-translational modification of pre-existing transcription factors.