Nicotinamide induces liver regeneration and improves liver function by activating SIRT1

Area of Science:

• Hepatology
• Regenerative Medicine
• Biochemistry

Background:

• Nicotinamide (Nam) shows pro-proliferation effects, indicating potential for tissue regeneration.
• The impact of Nicotinamide on liver regeneration is not yet understood.

Purpose of the Study:

• To evaluate Nicotinamide's efficacy in promoting liver regeneration and restoring function post-partial hepatectomy (PH).

Main Methods:

• Utilized C57BL/6 mice undergoing partial hepatectomy.
• Assessed hepatocyte proliferation using Ki-67 immunohistochemistry and cell cycle analysis.
• Evaluated liver function via H&E staining and serum liver injury markers.
• Measured NAD-dependent protein deacetylase sirtuin-1 (SIRT1) expression and activity through immunoblotting.

Main Results:

• Nicotinamide supplementation significantly promoted hepatocyte proliferation and accelerated liver tissue recovery.
• Nicotinamide protected liver function following PH, indicated by improved tissue morphology and reduced injury markers.
• Treatment with Nicotinamide led to a notable upregulation in SIRT1 expression and activity.

Conclusions:

• Nicotinamide effectively promotes liver regeneration and improves liver function post-PH in mice.
• Activation of SIRT1 by Nicotinamide is a likely mechanism underlying its regenerative effects.
• Nicotinamide shows potential as a therapeutic agent for enhancing liver regeneration after surgical resection.