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Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells

Courtney R Ogando1, Gilda A Barabino1, Yueh-Hsun Kevin Yang2

  • 1Department of Biomedical Engineering, Grove School of Engineering, The City University of New York - The City College, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2018
PubMed

Insights

This study details a protocol for differentiating aged human mesenchymal stem cells (MSCs) into bone and fat cells. It addresses challenges in expanding MSCs for regenerative medicine applications.

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Tissue engineering

Background:

  • Multipotent mesenchymal stem cells (MSCs) are crucial for tissue regeneration.
  • Extensive in vitro expansion leads to MSC senescence, reducing therapeutic potential.
  • Clinical applications require large numbers of MSCs, necessitating rapid expansion.

Purpose of the Study:

  • To provide a protocol for inducing adipogenic and osteogenic differentiation in aged human MSCs.
  • To identify and address potential issues during the differentiation of in vitro expanded MSCs.
  • To support the use of MSCs in regenerative medicine despite senescence challenges.

Main Methods:

  • A step-by-step protocol for adipogenic and osteogenic differentiation is described.
  • Focus on inducing differentiation in human MSCs that have undergone extensive in vitro expansion.
  • Identification of critical factors and potential pitfalls during the differentiation process.

Main Results:

  • The protocol facilitates adipogenic and osteogenic differentiation of aged human MSCs.
  • Key challenges encountered during the differentiation of senescent MSCs are highlighted.
  • The study provides insights into overcoming senescence-related limitations.

Conclusions:

  • Inducing differentiation in aged MSCs is feasible with a defined protocol.
  • Addressing senescence is critical for maximizing the therapeutic efficacy of MSCs.
  • This protocol aids in the application of MSCs for regenerative purposes.
Keywords:
AdipogenesisDifferentiationExpansionFlow cytometryHistologyMesenchymal stem cellOsteogenesisSenescence

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