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Protocols for Isolation and Evaluation of Muse Cells

Kazuki Tatsumi1,2, Yoshihiro Kushida1, Shohei Wakao1

  • 1Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan.

Advances in Experimental Medicine and Biology
|November 29, 2018
PubMed

Insights

This chapter details the isolation and pluripotency evaluation of Muse cells, derived from mesenchymal stem cells. It provides essential protocols for handling these multipotent stem cells for research applications.

Area of Science:

  • Stem Cell Biology
  • Cellular Reprogramming

Background:

  • Muse cells are multipotent stem cells with regenerative potential.
  • Mesenchymal stem cells (MSCs) serve as the basic population for Muse cell culture.
  • Standardized protocols are crucial for reliable Muse cell isolation and characterization.

Purpose of the Study:

  • To provide a detailed methodology for the isolation of Muse cells.
  • To outline methods for evaluating the pluripotency of isolated Muse cells.
  • To offer guidance on handling mesenchymal stem cells used in Muse cell culture.

Main Methods:

  • Isolation of Muse cells from various mesenchymal stem cell sources.
  • Assessment of pluripotency markers and functional assays.
  • Step-by-step protocols for cell culture and manipulation.

Main Results:

  • Successfully isolated Muse cells exhibiting pluripotency.
  • Established reliable methods for Muse cell characterization.
  • Provided a comprehensive protocol for MSC handling.

Conclusions:

  • The described methods enable efficient isolation and robust evaluation of Muse cell pluripotency.
  • This protocol serves as a valuable resource for researchers working with Muse cells and MSCs.
  • Standardized procedures ensure reproducibility in Muse cell research.
Keywords:
Adipose-derived stem cellsAnti-SSEA-3 antibodyCell sortingClustersFACSFibroblastsMSCsSerum lot check

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