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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Whole-cell biocatalysis platform for gram-scale oxidative dearomatization of phenols
Summer A Baker Dockrey1,2, Tyler J Doyon1,3, Jonathan C Perkins1,2
1Life Sciences Institute, University of Michigan, Ann Arbor, Michigan.
Abstract:
Technologies enabling new enzyme discovery and efficient protein engineering have spurred intense interest in the development of biocatalytic reactions. In recent years, whole-cell biocatalysis has received attention as a simple, efficient, and scalable biocatalytic reaction platform. Inspired by these developments, we have established a whole-cell protocol for oxidative dearomatization of phenols using the flavin-dependent monooxygenase, TropB. This approach provides a scalable biocatalytic platform for accessing gram-scale quantities of chiral synthetic building blocks.
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