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Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl-]i Decrease in Ciliated Human Nasal

Taka-Aki Inui1,2, Makoto Yasuda3, Shigeru Hirano4

  • 1Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. inui1227@koto.kpu-m.ac.jp.

International Journal of Molecular Sciences
|November 30, 2018
PubMed
Summary
This summary is machine-generated.

The isoflavone daidzein enhances ciliary beat distance (CBD) in human nasal cells by activating chloride channels, which improves airway mucus clearance. This finding suggests daidzein may benefit respiratory health.

Keywords:
amplitude of ciliary beatingciliaintracellular Cl− concentrationmucociliary clearancenasal epithelia

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Area of Science:

  • Cell Biology
  • Respiratory Physiology
  • Pharmacology

Background:

  • Cilia play a vital role in mucociliary clearance in the airways.
  • Ciliary function is influenced by intracellular ion concentrations, particularly chloride.
  • Isoflavones, like daidzein, are plant-derived compounds with potential biological activities.

Purpose of the Study:

  • To investigate the effects of the isoflavone daidzein on ciliary beat distance (CBD) and ciliary beat frequency (CBF).
  • To elucidate the role of intracellular chloride ([Cl⁻]i) in mediating daidzein's effects on ciliary function.
  • To assess the impact of daidzein-induced changes in ciliary function on airway transport.

Main Methods:

  • Primary culture of ciliated human nasal epithelial cells (cHNECs).
  • Measurement of intracellular chloride ([Cl⁻]i), ciliary beat distance (CBD), and ciliary beat frequency (CBF).
  • Pharmacological manipulation using chloride-free solutions, bumetanide, and NPPB (chloride channel blocker).
  • Microbead transport assays to evaluate ciliary transport efficiency.

Main Results:

  • Daidzein decreased intracellular chloride ([Cl⁻]i) and enhanced ciliary beat distance (CBD) in cHNECs at 37°C.
  • The effects of daidzein on CBD were mimicked by conditions that reduce [Cl⁻]i and inhibited by a chloride channel blocker.
  • Daidzein enhanced microbead transport, indicating improved ciliary clearance.
  • Intracellular chloride affects CBD and CBF in a temperature-dependent manner.

Conclusions:

  • Daidzein activates chloride channels in cHNECs, leading to decreased intracellular chloride and increased CBD.
  • Increased CBD, stimulated by daidzein, enhances ciliary transport in the airways.
  • These findings highlight the potential of daidzein in modulating ciliary function for improved respiratory health.