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Related Experiment Videos

Low affinity E-rosette formation by the human K cell.

W H West, R B Boozer, R B Herberman

    Journal of Immunology (Baltimore, Md. : 1950)
    |January 1, 1978
    PubMed
    Summary
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    Tissue distribution of adoptively transferred adherent LAK cells: role of the route of administration.

    Natural immunity·1992

    Human K cells, responsible for antibody-dependent cell-mediated cytotoxicity (ADCC), are primarily found within a specific subset of T lymphocytes identified by low-affinity E-rosette formation. This finding helps predict cytotoxic potential in peripheral blood.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human T lymphocytes can be separated into subsets based on their affinity for sheep red blood cells (E).
    • Antibody-dependent cell-mediated cytotoxicity (ADCC) is a key immune function mediated by cytotoxic cells, often referred to as K cells.

    Purpose of the Study:

    • To investigate the relationship between T lymphocyte subsets, defined by E-rosette formation affinity, and their cytotoxic activity in ADCC.
    • To identify the specific T cell subset responsible for ADCC.

    Main Methods:

    • Separation of human T lymphocytes into high and low affinity E-rosette-forming cells (E-RFC).
    • Assay of cytotoxic reactivity of these subsets against antibody-sensitized target cells (liver cells).
    • Correlation analysis between E-RFC proportions and ADCC activity in peripheral blood.

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    Main Results:

    • High affinity E-RFC, prevalent in thymus, tonsil, and lymph node, showed minimal ADCC activity.
    • Low affinity E-RFC, found predominantly in peripheral blood and spleen, were highly enriched for ADCC activity.
    • A strong correlation was observed between the proportion of low affinity E-RFC in peripheral blood and ADCC reactivity.

    Conclusions:

    • Human K cells mediating ADCC are predominantly found within the low affinity E-RFC subset.
    • The E-rosette formation pattern can predict the cytotoxic potential of peripheral blood mononuclear cells.
    • This suggests a distinct, functionally significant subset of thymic-dependent mononuclear cells involved in cell-mediated immunity.