Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Blood Typing01:10

Blood Typing

2.8K
Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target...
2.8K
Blood Types02:20

Blood Types

22.9K
Human blood is classified into different types based on the presence of antigens on the red blood cell's surface and antibodies in the plasma. Proper identification of blood type is essential for successful blood transfusion. The International Society of Blood Transfusion has identified 38 human blood types based on the surface antigens on the red blood cells. The most common types are ABO, Rh, and MNS blood types.
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...
22.9K
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

9.3K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
9.3K
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

27.4K
Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
27.4K
Resonance02:52

Resonance

65.4K
The Lewis structure of a nitrite anion (NO2−) may actually be drawn in two different ways, distinguished by the locations of the N-O and N=O bonds.
65.4K
Imaging Studies IV: Magnetic Resonance Imaging01:27

Imaging Studies IV: Magnetic Resonance Imaging

278
Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...
278

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Allotypes matter: Evaluation of twelve commercially available antihuman IgG2 antibodies for subclass specificity.

Journal of immunological methods·2026
Same author

Essential but neglected: securing the global supply of human hyperimmune immunoglobulins.

The Lancet. Haematology·2026
Same author

Fc-fucosylation of Plasmodium falciparum-specific IgG varies with antigen and immunization.

The Journal of infectious diseases·2026
Same author

An RHD gene variant, common in southern Chinese Han, involves a short 688 bp deletion and a long 21.8 kb insertion in the RHD exon 10 non-coding region, affecting the accuracy of red cell genotyping.

Journal of translational medicine·2026
Same author

Structural promiscuity in the human circulatory IgA1 clonal repertoire.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Adverse perinatal outcomes indicative of RhD-mediated hemolytic disease of the fetus and newborn in Eastern Ethiopia: evidence of maternal health inequity in a multicenter cohort study.

AJOG global reports·2026

Related Experiment Video

Updated: Feb 1, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

2.0K

Multiplex blood group typing by cellular surface plasmon resonance imaging.

Zoltan Szittner1, Arthur E H Bentlage1, Eric van der Donk2

  • 1Department of Experimental Immunohematology, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.

Transfusion
|November 30, 2018
PubMed
Summary

Surface plasmon resonance (SPR) enables rapid, simultaneous blood typing for multiple antigens. This method accurately matches blood donors and patients, improving transfusion safety and efficiency.

More Related Videos

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.8K
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
10:41

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

13.8K

Related Experiment Videos

Last Updated: Feb 1, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

2.0K
Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.8K
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
10:41

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

13.8K

Area of Science:

  • Biotechnology
  • Immunology
  • Medical Diagnostics

Background:

  • Accurate blood-group typing is critical to prevent transfusion complications like alloimmunization.
  • Current methods struggle with high-throughput multiplex typing of numerous blood group antigens.
  • Identifying compatible blood is challenging due to over 300 known blood group antigens.

Purpose of the Study:

  • To develop and validate a Surface Plasmon Resonance (SPR) array for simultaneous, high-throughput blood typing.
  • To assess the efficacy of SPR in accurately identifying key blood group antigens (A, B, Rh).
  • To compare SPR-based typing with conventional agglutination methods for clinical relevance.

Main Methods:

  • Utilized SPR, a label-free technology, to monitor real-time interactions between red blood cells (RBCs) and immobilized blood group antibodies.
  • Developed an SPR array functionalized with clinically relevant antibodies for A, B, and Rh blood groups.
  • Blindly compared SPR typing results for 946 blood donors against standard agglutination-based serology.

Main Results:

  • Achieved simultaneous RBC typing for multiple antigens within 5 minutes per sample.
  • Demonstrated robust SPR chip regeneration, enabling typing of at least 100 samples.
  • Obtained a 100% match with classical serology for tested antibodies, except for inconsistent anti-E/e results due to antibody specificity issues.

Conclusions:

  • SPR-based blood typing is a viable method for simultaneous, multiplex antigen detection.
  • This approach offers reduced hands-on time and potential cost efficiencies for blood transfusion services.
  • High-quality, specific antibodies are crucial for the accuracy of SPR-based blood typing systems.