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Genomewide Transcriptome Profiles Reveal How Bacillus subtilis Lipopeptides Inhibit Microsclerotia Formation in

Dimei Yu1, Yulin Fang1, Chen Tang1

  • 11 Beijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing, China; and.

Molecular Plant-Microbe Interactions : MPMI
|November 30, 2018
PubMed

Insights

Bacillus subtilis lipopeptides effectively suppress microsclerotia formation in Verticillium dahliae, a key factor in vascular wilt diseases. This study reveals the molecular mechanisms, paving the way for new biological controls.

Area of Science:

  • Plant Pathology
  • Microbiology
  • Biochemistry

Background:

  • Verticillium dahliae causes devastating vascular wilt diseases globally.
  • Melanized microsclerotia are critical for V. dahliae survival and spread.
  • Effective and eco-friendly fungicides against V. dahliae are lacking.

Purpose of the Study:

  • To elucidate the mechanism by which Bacillus subtilis C232 lipopeptides suppress V. dahliae microsclerotia formation.
  • To identify the molecular targets of these lipopeptides in V. dahliae.

Main Methods:

  • Liquid chromatography-mass spectrometry identified antifungal compounds as lipopeptides.
  • Exposure of V. dahliae to lipopeptides and subsequent transcriptomic analysis (RNA sequencing).
  • Gene ontology enrichment analysis to identify affected pathways.

Main Results:

  • Lipopeptides caused hyphal swelling, cell lysis, and downregulated melanin synthesis genes in V. dahliae.
  • Transcriptomic analysis revealed differential expression of 5,974 genes.
  • Enrichment analysis highlighted impacts on stress response, metabolism, translation, and specific signaling pathways.

Conclusions:

  • B. subtilis lipopeptides disrupt V. dahliae microsclerotia formation via multiple molecular pathways.
  • Understanding these mechanisms supports the development of novel biological control agents for Verticillium wilt.

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