Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human thymus-leukemia-associated antigen: isolation and partial characterization.

B E Chechik, M E Percy, E W Gelfand

    Journal of the National Cancer Institute
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Antibody-directed therapy: past, present, and future.

    The Journal of allergy and clinical immunology·2001
    Same author

    gammadelta T cells as regulators of airway hyperresponsiveness.

    International archives of allergy and immunology·2001
    Same author

    Strain dependence of airway hyperresponsiveness reflects differences in eosinophil localization in the lung.

    American journal of physiology. Lung cellular and molecular physiology·2001
    Same author

    Accumulation of high levels of the p53 and p130 growth-suppressing proteins in cell lines stably over-expressing cyclin-dependent kinase 6 (cdk6).

    Oncogene·2001
    Same author

    Pulmonary T cells and eosinophils: coconspirators or independent triggers of allergic respiratory pathology?

    The Journal of allergy and clinical immunology·2001
    Same author

    Preferential binding of Staphylococcus aureus to skin sites of Th2-mediated inflammation in a murine model.

    The Journal of investigative dermatology·2001
    Same journal

    Racial and ethnic disparities in clinical outcomes of HER2-positive metastatic breast cancer treated with antibody-drug conjugates.

    Journal of the National Cancer Institute·2026
    Same journal

    Breast cancer risk prediction with artificial intelligence: promise, calibration, and clinical readiness.

    Journal of the National Cancer Institute·2026
    Same journal

    RE：Electroacupuncture improves cognitive function and neuropsychiatric symptoms in breast cancer survivors: a pilot randomized controlled trial.

    Journal of the National Cancer Institute·2026
    Same journal

    Worsening frailty and declining quality of life in older survivors of non-metastatic breast cancer.

    Journal of the National Cancer Institute·2026
    Same journal

    Longitudinal effects of comorbidities on brain structure and cognition in older breast cancer survivors.

    Journal of the National Cancer Institute·2026
    Same journal

    Response to Wang et al. and Shen et al.

    Journal of the National Cancer Institute·2026
    See all related articles

    Researchers isolated two forms of the Human thymus-leukemia-associated antigen (HThy-L) from human thymus tissue. The 43,000-dalton component of HThy-L was found to possess antigen activity.

    Area of Science:

    • Immunology
    • Biochemistry
    • Cell Biology

    Background:

    • Human thymus-leukemia-associated antigen (HThy-L) is a saline-soluble antigen.
    • HThy-L has been detected in thymocytes, T-cell lines, and leukemia blasts.

    Purpose of the Study:

    • To identify and isolate HThy-L species from normal human thymus tissue.
    • To characterize the molecular properties of HThy-L.

    Main Methods:

    • Extraction and purification using tris buffer, ammonium sulfate fractionation, acid precipitation, DEAE-cellulose chromatography, Sephadex G-100 gel filtration, and carboxymethyl-cellulose chromatography.
    • Molecular weight determination using Sephadex G-100 gel filtration and SDS-PAGE.
    • Activity assessment via Sephadex G-50 purification.

    Related Experiment Videos

    Main Results:

    • Two species of HThy-L were isolated from human thymus.
    • Both HThy-L species exhibited a molecular weight of 40,000–50,000 on Sephadex G-100.
    • SDS-PAGE revealed two components (approx. 43,000 and 23,000 daltons) in each HThy-L species.
    • The 43,000-dalton component demonstrated HThy-L activity.

    Conclusions:

    • The study successfully isolated and characterized two species of HThy-L.
    • The 43,000-dalton component is identified as the active moiety of HThy-L.