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Proteolytic activity in electropherograms (SDS-PAGE) of bovine erythrocyte ghosts
1Laboratory of Biophysics of Development and Aging, University of Lódź, Poland.
The International Journal of Biochemistry
|January 1, 1988
Abstract:
1. Activity of proteases, strongly related with erythrocyte membrane, was analysed employing a new methodological approach. 2. Intact bovine ghosts, ghosts depleted in peripheric proteins or purified Triton X-100 and ghost extracts were electrophoresed and the proteolytic activity in the gel fragments (SDS-PAGE) was assayed. 3. At least two proteases that were inhibited by EDTA and PMSF were found.