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Very short patch mismatch repair activity associated with gene dcm is not conferred by a plasmid coding for EcoRII
1Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033.
Journal of Bacteriology
|October 1, 1988
Abstract:
The only cytosine methylase in Escherichia coli K-12 methylates the second cytosine in the sequence CC (A/T)GG and is encoded by gene dcm. Methylation and very short patch mismatch repair activities lacking in a dcm mutant of E. coli were restored by a plasmid containing the cloned dcm gene. In contrast, plasmids with the gene for EcoRII methylase, which is a homolog of dcm, restored only cytosine methylase activity and not mismatch repair.