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Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II
C Handl1, B Rönnberg, B Nilsson
1Center for Biotechnology, F82 Huddinge University Hospital, Sweden.
Journal of Clinical Microbiology
|August 1, 1988
Abstract:
The gene for Escherichia coli heat-stable enterotoxin type II (STII) was fused to the genes for protein A from Staphylococcus aureus and beta-galactosidase in two different expression systems. Antibodies raised in rabbits against the protein A-STII fusion protein recognized the beta-galactosidase-STII fusion protein. The latter fusion protein was used as the immobilized antigen in an enzyme-linked immunosorbent assay (ELISA) for detection of STII. The correlation between the results of the ELISA and the intestinal loop test in piglets was 95%, suggesting that the ELISA can be used to reliably detect STII.