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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
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Sonic hedgehog drives layered double hydroxides-induced acute inflammatory landscape
Geórgia da Silva Feltran1, Célio Junior da Costa Fernandes1, Marcel Rodrigues Ferreira1
1Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, campus Botucatu, São Paulo, CEP 18618-970, Brazil.
Colloids and Surfaces. B, Biointerfaces
|November 30, 2018
Summary
Layered double hydroxides (LDHs) modulate osteoblast inflammation by down-regulating pro-inflammatory genes and up-regulating anti-inflammatory ones. Sonic hedgehog (Shh) signaling is crucial for these anti-inflammatory responses.
Area of Science:
- Biomaterials Science
- Nanomedicine
- Cell Biology
Background:
- Layered double hydroxides (LDHs) show promise in healthcare but their effects on osteoblast inflammatory signaling are understudied.
- Osteoblasts play a critical role in bone health and inflammation.
Purpose of the Study:
- To investigate the inflammatory signaling of osteoblasts in response to LDHs.
- To determine the influence of Sonic hedgehog (Shh) signaling on LDH-induced osteoblast responses.
Main Methods:
- Osteoblasts were treated with Mg2Al-Cl and Zn2Al-Cl LDHs.
- Shh signaling was inhibited using cyclopamine (cyc).
- Gene expression of inflammasomes and cytokines (pro- and anti-inflammatory) was analyzed.
Main Results:
- Zn2Al-Cl LDHs upregulated inflammasome-related genes (asc1, nrlp3).
- LDHs down-modulated pro-inflammatory cytokine genes (tnfα, il1ß) and up-modulated anti-inflammatory cytokine genes (il13, il10, tgfß), except il6.
- Shh inhibition further enhanced the upregulation of il13 and il10.
Conclusions:
- LDHs induce a shift in osteoblast inflammatory signaling towards an anti-inflammatory profile.
- Shh signaling plays a significant role in mediating these anti-inflammatory effects of LDHs on osteoblasts.
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