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Related Experiment Video

Updated: Feb 1, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

GLOSSary: the GLobal Ocean 16S subunit web accessible resource.

M Tangherlini¹, M Miralto², C Colantuono²

¹Stazione Zoologica "Anton Dohrn", Villa Comunale, 80121, Naples, Italy. michael.tangherlini@szn.it.

BMC Bioinformatics

|December 1, 2018

Summary

This summary is machine-generated.

This study introduces GLOSSary, a bioinformatics platform for managing and analyzing environmental metagenomics data from the Tara Ocean Project. It enables efficient exploration of 16S rDNA sequences and associated metadata, supporting broader scientific research.

Keywords:

16S rRNA Marine metagenomics Tara Ocean project Taxonomic analysis

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Published on: July 11, 2016

Area of Science:

Bioinformatics
Environmental Metagenomics
Oceanography

Background:

Environmental metagenomics generates vast sequence data requiring advanced computational tools.
Bioinformatics is crucial for managing, processing, and mining this data, especially from large projects like the Tara Ocean Project.

Purpose of the Study:

To present novel computational approaches for handling complex metagenomics data.
To develop user-friendly web platforms for integrating environmental metagenomics sequences with metadata.
To create a bioinformatics platform for analyzing 16S rDNA sequences using reference databases.

Main Methods:

Processed Tara 16S miTAGs, generating assembled 16S rDNA sequences.
Classified sequences based on length, chimeric reads, and taxonomic affiliation.
Organized data in a NoSQL database with geospatial query capabilities.
Developed a web interface for interactive data exploration and visualization.

Main Results:

Included processed Tara 16S data in GLOSSary (the GLobal Ocean 16S Subunit web accessible resource).
Enabled exploration of taxonomic affiliations and spatial distribution of sequence data.
Provided access to raw miTAG reads, 16S contigs, and unassembled sequences.
Integrated data with the SILVA database for BLAST analysis.

Conclusions:

GLOSSary offers an expandable bioinformatics environment for environmental metagenomics.
The platform supports current and future analyses by the scientific community.