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Updated: Feb 1, 2026
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
Published on: March 5, 2019
MiR-1-5p is down-regulated in gallbladder carcinoma and suppresses cell proliferation, migration and invasion by
Chun Bo Hua1, Sheng Bo Song2, Hui Li Ma3
1General Surgery Ward One, The Fifth Hospital of Harbin, Harbin, Heilongjiang, 150040, China.
Background:
Numerous studies have demonstrated that aberrant microRNAs (miRNAs) are involved in tumorigenesis and tumor progression. Nevertheless, the precise role of miR-1-5p in gallbladder carcinoma cell growth and metastasis remains not fully revealed.
Material And Methods:
The levels of miR-1-5p were detected in gallbladder carcinoma tissues and cell lines using qRT-PCR method. A series of functional assays, including cell proliferation, colony formation, wound healing and Transwell invasion were conducted using miR-1-5p or miR-1-5p inhibitor transfected cells.
Results:
MiR-1-5p was remarkably down-regulated in gallbladder carcinoma tissues and cell lines compared to normal. In addition, over-expression of miR-1-5p markedly suppressed the growth, migration and invasion of gallbladder carcinoma cell. Conversely, down-expression of miR-1-5p facilitated gallbladder carcinoma cell proliferation and aggressiveness. Mechanistic investigations demonstrated that neurogenic locus notch homolog protein 2 (Notch2) was the directly target of miR-1-5p and Notch2 mediated the inhibitory effect of miR-1-5p in gallbladder carcinoma cell growth and aggressiveness.
Conclusion:
Our findings demonstrated that miR-1-5p acted as a suppressive miRNA and played vital roles in the growth, migration and invasion of gallbladder carcinoma cell through targeting Notch2.
Insights
MicroRNA-1-5p (miR-1-5p) is down-regulated in gallbladder cancer, suppressing tumor growth and metastasis. Targeting Notch2, miR-1-5p acts as a tumor suppressor in this cancer.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Aberrant microRNAs (miRNAs) are implicated in cancer development.
- The specific role of miR-1-5p in gallbladder carcinoma is not fully understood.
Purpose of the Study:
- To investigate the role of miR-1-5p in gallbladder carcinoma.
- To identify the molecular mechanisms underlying miR-1-5p's function in this cancer.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure miR-1-5p levels.
- Functional assays including cell proliferation, colony formation, migration, and invasion assays.
- Transfection with miR-1-5p mimics or inhibitors.
Main Results:
- MiR-1-5p expression was significantly reduced in gallbladder carcinoma tissues and cell lines.
- Overexpression of miR-1-5p inhibited cell growth, migration, and invasion.
- Downregulation of miR-1-5p promoted proliferation and aggressiveness.
- Neurogenic locus notch homolog protein 2 (Notch2) was identified as a direct target of miR-1-5p.
Conclusions:
- MiR-1-5p functions as a tumor suppressor in gallbladder carcinoma.
- MiR-1-5p inhibits cancer cell growth, migration, and invasion by targeting Notch2.
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