Related Experiment Video

Updated: Feb 1, 2026

A Piglet Perinatal Asphyxia Model to Study Cardiac Injury and Hemodynamics after Cardiac Arrest, Resuscitation, and the Return of Spontaneous Circulation
10:55

A Piglet Perinatal Asphyxia Model to Study Cardiac Injury and Hemodynamics after Cardiac Arrest, Resuscitation, and the Return of Spontaneous Circulation

Published on: January 13, 2023

2.3K

Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia

Valentina Vio1,2, Ana L Riveros1, Andrea Tapia-Bustos2

  • 1Department of Pharmacological and Toxicology Chemistry, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile, mkogan@ciq.uchile.cl.

International Journal of Nanomedicine
|December 1, 2018
PubMed

Insights

This study developed a novel nanosystem to deliver siRNA, effectively silencing poly (ADP-ribose) polymerase 1 (PARP-1) in neonatal rats. This targeted gene silencing offers a promising therapeutic strategy for mitigating perinatal asphyxia effects.

Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Genetics

Background:

  • Perinatal asphyxia causes significant neonatal developmental deficits and long-term neurological issues.
  • Overactivation of poly (ADP-ribose) polymerase 1 (PARP-1) exacerbates energy crises in asphyxia models.
  • PARP-1 inhibition presents a potential therapeutic avenue for perinatal asphyxia.

Purpose of the Study:

  • To develop and evaluate a targeted nanosystem for delivering siRNA to inhibit PARP-1.
  • To assess the efficacy of brain-targeted PARP-1 gene silencing in an in vivo model of perinatal asphyxia.

Main Methods:

  • A nanosystem was engineered using gold nanorods (AuNR) conjugated with CLPFFD peptide for brain targeting.
  • The nanosystem encapsulated siRNA specifically designed for PARP-1 knockdown.
  • In vitro delivery and gene silencing in PC12 cells were confirmed, followed by in vivo administration in asphyxia-exposed rat pups.

Main Results:

  • Efficient delivery of siRNA into PC12 cells, leading to successful gene silencing.
  • Demonstrated ability of the AuNR-CLPFFD/siRNA complex to reach the brain in vivo.
  • Effective inhibition of PARP-1 in the brain of asphyxia-exposed rat pups.

Conclusions:

  • The developed nanosystem effectively delivers siRNA for targeted gene silencing.
  • This approach successfully inhibited PARP-1 in vivo, demonstrating therapeutic potential.
  • The combination of targeted delivery and gene silencing offers a novel strategy for perinatal asphyxia treatment.
Abstract

Keywords:
PARP-1 knockdownPC12gold nanorodsin vivo administrationneonatal hypoxiaratssiRNA delivery

More Related Videos

Hydroquinone Based Synthesis of Gold Nanorods08:55

Hydroquinone Based Synthesis of Gold Nanorods

Published on: August 10, 2016

14.9K
Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates09:17

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Published on: March 5, 2019

9.2K

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.6K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

4.6K
Standard Electrode Potentials03:02

Standard Electrode Potentials

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.3K
Cell Potential and Free Energy02:58

Cell Potential and Free Energy

Thermodynamics of a Redox Reaction
Thermodynamics is the branch of physics dealing with the relationship between heat and other forms of energy. In an electrochemical cell, chemical energy is converted into electrical energy.
Thus, a link can be predicted between cell potential, free energy change, and the equilibrium constant for the reaction. Cell potential can also be measured as the oxidant or the reducing strength, and similar acid-base strength measures are reflected in equilibrium...
46.5K
Potential Energy00:52

Potential Energy

The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.7K
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K