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Updated: Feb 1, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Aqueous extracts of Agave sisalana boles have prebiotic potential
Alexsandra Conceição Apolinário1,2, Antonio Diogo Silva Vieira1, Susana Marta Isay Saad1
1Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, 05508-000, Brazil.
Abstract:
This work aimed at evaluating the prebiotic potential of the aqueous extract and crude polysaccharides from Agave sisalana boles by an in vitro screening. Crude polysaccharides were obtained from the aqueous bole extract by precipitation with acetone and resuspension in water. The liquid extract and the polysaccharide solution were then spray dried and submitted to thermal analysis and quantification of metabolites. Prebiotic activity was checked on probiotic strains belonging to the Lactobacillus genus using inulin, fructo-oligosaccharides, fructose and glucose as positive controls. The powder of A. sisalana bole extract, which has recently been identified as a rich source of inulin, exhibited higher potential of fermentation compared with crude polysaccharides.
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