Nanozymes in Nanofibrous Mats with Haloperoxidase-like Activity To Combat Biofouling

Electrospun polymer mats are widely used in tissue engineering, wearable electronics, and water purification. However, in many environments, the polymer nanofibers prepared by electrospinning suffer from biofouling during long-term usage, resulting in persistent infections and device damage. Herein, we describe the fabrication of polymer mats with CeO_{2- x} nanorods that can prevent biofouling in an aqueous environment. The embedded CeO_{2- x} nanorods are functional mimics of natural haloperoxidases that catalyze the oxidative bromination of Br^- and H₂O₂ to HOBr. The generated HOBr, a natural signaling molecule, disrupted the bacterial quorum sensing, a critical step in biofilm formation. The polymer fibers provide porous structures with high water wettability, and the embedded cerium oxide nanozymes act as a catalyst that can efficiently trigger oxidative bromination, as shown by a haloperoxidase assay. Additionally, the embedded nanozymes enhance the mechanical property of polymer mats, as shown by a single-fiber bending test using atomic force microscopy. We envision that the fabricated polymer mats with CeO_{2- x} nanorods may be used to provide mechanically robust coatings with antibiofouling properties.