ATP-Concentration- and Force-Dependent Chemomechanical Coupling of Kinesin Molecular Motors

A model is presented for the chemomechanical coupling of kinesin motors, which proposes that the rate constants of the chemical reaction are independent of the external force. On the basis of the model, we study theoretically the movement dynamics of the motors under varying external force and ATP concentration, such as the forward to backward stepping ratio, velocity, dwell time between two mechanical steps, stall force, and so on. The theoretical results reproduce quantitatively the diverse and even contradictory available single-molecule experimental data for different species of the motors. Furthermore, we study the dependence of the chemomechanical coupling ratio on ATP concentration and external force, with both ATP concentration and external force having large effects on the chemomechanical coupling.