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Trace detection of some nitro-explosives using thermal mediated immunochemical defragmented method.

Shilpa Chaudhary1, Praveen Sonkusre2, K K Bhasin3

  • 1CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India; Department of Chemistry, Panjab University, Chandigarh 160014, India.

Biosensors & Bioelectronics
|December 1, 2018
PubMed
Summary

A novel immunoassay detects explosives like TNT, RDX, and PETN using thermal lysis. This method generates nitrite ions for sensitive detection in mixed samples.

Keywords:
Colorimetric immunoassayGriess reagentHapten designNitro-explosiveSpecific antibodiesThermolysis

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Immunochemistry

Background:

  • Nitro-explosives pose significant security and safety risks.
  • Existing detection methods often lack specificity and speed.
  • There is a need for rapid, selective detection of explosive compounds.

Purpose of the Study:

  • To develop a novel immunoassay for selective detection of nitro-explosives.
  • To utilize thermally induced defragmentation for explosive analysis.
  • To quantify 2,4,6-trinitrotoluene (TNT), 1,3,5-trinitroperhydro-1,3,5-triazine (RDX), and pentaerythritol tetranitrate (PETN).

Main Methods:

  • Generation of specific antibodies against TNT, RDX, and PETN using designed haptens.
  • Development of a thermal mediated immunochemical biosensing technique.
  • Formation of immunocomplexes, followed by thermal lysis to produce nitrite ions.
  • Quantification of nitrite ions using Griess reagent to form a colored chromophore.

Main Results:

  • Successful generation of specific antibodies for target nitro-explosives.
  • Demonstration of a highly selective immunoassay format.
  • Establishment of a correlation between chromophore color intensity and explosive concentration.
  • Effective detection of explosives from a mixture pool.

Conclusions:

  • The developed immunoassay offers a highly specific and rapid method for detecting nitro-explosives.
  • Thermal lysis coupled with immunochemical detection provides a sensitive analytical approach.
  • This technique addresses the need for improved explosive detection systems.