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Related Experiment Videos

Phospholipase A2 activity associated with synovial fluid cells.

W Pruzanski1, P Vadas, J Kim

  • 1Immunology Diagnostic and Research Centre, University of Toronto, Wellesley Hospital, Canada.

The Journal of Rheumatology
|January 1, 1988
PubMed
Summary

Synovial fluid cells from inflammatory arthritis patients exhibit high phospholipase A2 (PLA2) activity. This enzyme can also passively adsorb to peripheral blood neutrophil membranes from synovial fluid, indicating a potential source of inflammation.

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Area of Science:

  • Biochemistry
  • Immunology
  • Rheumatology

Background:

  • High phospholipase A2 (PLA2) activity is observed in synovial fluids (SF) of inflammatory arthritides.
  • The cellular source of SF PLA2 remains unidentified.

Purpose of the Study:

  • To investigate the presence and origin of PLA2 within SF cells.
  • To determine if PLA2 can adsorb to peripheral blood neutrophils.

Main Methods:

  • PLA2 content was measured in sonicates of SF cells and peripheral blood neutrophils (PMN).
  • Spontaneous release of PLA2 from unstimulated cells was assessed.
  • PMN were incubated with SF or purified PLA2 to evaluate passive adsorption.

Main Results:

  • SF cells showed significantly higher PLA2 activity compared to normal peripheral blood PMN.

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  • SF cells released PLA2 spontaneously, unlike PMN.
  • Soluble PLA2 readily adsorbed to PMN membranes in a concentration-dependent manner.

    • Conclusions:

    • SF cells are a significant source of PLA2 in inflammatory arthritis.
    • PLA2 can adsorb to circulating neutrophils, potentially contributing to joint inflammation.
    • PLA2 adsorption to PMN warrants further investigation in arthritic conditions.