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[Identification of Phenotype and Genotype in One Case with Weak D blood group]
Hui-Ying Xu1, Rui Wang2, Chun-Yan Lin3
1Department of Laboratorial Medicine,The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China.
Objective:
To investigate the phenotype and genotype of the weak D blood group in one case of Chinese Han people.
Methods:
Phenotype of blood sample was identified with serologic tests; PCR-SBT was applied for the analysis of genotype and RhD zygosity.
Results:
Both saline and gel card tests demonstrated this case to be dCcee, which was contrary to glass bead card result. Some of the RBC D epitopes were negative.c.1022T>A allele was identified with PCR-SBT and the zygosity analysis showed this case to be D/d.
Conclusion:
RHD*1022 A is more suitable to be categorized as weak partial D other than weak D in a Chinese Han people.
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