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TRMT2A is a novel cell cycle regulator that suppresses cell proliferation
Yu-Hsin Chang1, Susumu Nishimura2, Hisashi Oishi3
1Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan; Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Ibaraki, Japan.
Biochemical and Biophysical Research Communications
|December 4, 2018
Summary
Mammalian tRNA methyltransferase 2 homolog A (TRMT2A) inhibits cell proliferation and controls the cell cycle. This study identifies TRMT2A as a potential regulator of cell cycle progression in mammals.
Area of Science:
- Molecular Biology
- Biochemistry
- Cell Biology
Background:
- Transfer RNA (tRNA) undergoes post-transcriptional chemical modifications for maturation.
- 5-Methyluridine (m5U) is a conserved tRNA modification found across species.
- The mammalian enzyme responsible for m5U54 tRNA methylation is largely uncharacterized.
Purpose of the Study:
- To characterize the mammalian TRMT2A protein, an enzyme involved in tRNA methylation.
- To investigate the biological function and cellular role of TRMT2A.
Main Methods:
- Protein domain analysis of TRMT2A.
- Subcellular localization studies in HeLa cells.
- Analysis of cell proliferation and cell cycle in TRMT2A-overexpressing and deficient cells.
Main Results:
- TRMT2A possesses an RNA recognition motif and a methyltransferase domain.
- TRMT2A localizes primarily to the nucleus.
- TRMT2A overexpression reduces cell proliferation and alters DNA content, while TRMT2A deficiency enhances growth.
Conclusions:
- TRMT2A acts as an inhibitor of mammalian cell proliferation.
- TRMT2A plays a role in cell cycle control.
- TRMT2A is identified as a potential mammalian cell cycle regulator.
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