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Updated: Feb 1, 2026

Cell-Free Dot Blot as a Practical and Adaptable Immunoassay Platform for the Detection of Antibody Response in Human and Animal Sera
Published on: May 23, 2025
Silver enhanced nano-gold dot blot immunoassay for leptospirosis
Veerapandian Raja1, Muthu Prasad1, Palanisamy Bothammal1
1Medical Microbiology Laboratory, Department of Microbiology, Center for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Abstract:
Leptospirosis is a widespread zoonotic disease and lacks in efficient diagnostic tools. In the present study, a nanogold based dot blot immunoassay was developed and evaluated for the detection of leptospirosis in human urine samples. This method was found to be rapid (<4 h) with higher sensitivity (>4.2-14.6%) than horse radish peroxidase (HRP) conjugated dot blot assay.
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