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Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
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Relationship between nasal Carrier isolates and clinical isolates in children with Staphylococcus aureus infections

Shan Tan1, Chaomin Wan1, Hongren Wang2

  • 1Department of Pediatrics, West China Second Hospital, Sichuan University and Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, No. 20, 3rd Section of Renmin South Road, Chengdu, 610041, China.

Microbial Pathogenesis
|December 4, 2018
PubMed

Insights

Staphylococcus aureus nasal carriage in children strongly correlates with infection sites, with ST59 being a dominant, virulent MRSA clone. Eliminating nasal carriage may prevent systemic S. aureus infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Staphylococcus aureus (S. aureus) infections are a significant concern in pediatric populations.
  • Understanding the relationship between nasal colonization and infection is crucial for effective treatment and prevention.

Purpose of the Study:

  • To investigate the genetic relationship between S. aureus strains found in the anterior nares (colonization) and those causing infections at other body sites in children.
  • To characterize key virulence factors and genetic profiles of these strains.

Main Methods:

  • Polymerase chain reaction (PCR) was used to detect the methicillin resistance (mecA) and Panton-Valentine leukocidin (PVL) genes.
  • Multilocus sequence typing (MLST) was employed to determine the sequence types (STs) of S. aureus isolates from nasal and non-nasal sites in 56 hospitalized children.
  • 22 pairs of isolates were analyzed.

Main Results:

  • Sequence Type 59 (ST59) was identified as the predominant clonal type in nasal S. aureus isolates.
  • ST59 isolates showed statistically significant differences in mecA and PVL gene expression compared to other STs.
  • A high degree of consistency (86.4%) was observed between nasal and non-nasal isolates regarding mecA, PVL, and ST profiles, indicating a strong correlation (Kappa = 0.950).

Conclusions:

  • ST59 is a dominant and virulent methicillin-resistant S. aureus (MRSA) clone in the Sichuan region, potentially leading pediatric MRSA infections.
  • A strong correlation exists between S. aureus nasal colonization and infection in pediatric patients, with isolates often being genetically indistinguishable and endogenous.
  • Routine nasal swabs and potential elimination of nasal carriage could be considered as a preventive strategy for systemic S. aureus infections in children.
Abstract

Keywords:
ChildrenClinical infectionsNasal colonizationStaphylococcus aureus

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