Performance and kinetics of triclocarban removal by entrapped Pseudomonas fluorescens strain MC46
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Updated: Feb 1, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
Pongsatorn Taweetanawanit1, Thunyalux Ratpukdi2, Sumana Siripattanakul-Ratpukdi2
1Department of Environmental Engineering, Faculty of Engineering and Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand.
Abstract:
This study investigated removal of triclocarban (TCC) from contaminated wastewater by Pseudomonas fluorescens strain MC46 entrapped in barium alginate. Appropriate entrapped cell preparation conditions (cell-to-entrapment material ratio and cell loading) for removing TCC were examined. The highest TCC removal by the entrapped and free cell systems at the initial TCC concentration of 10 mg/L was 72 and 45%, respectively. TCC was degraded to less toxic compounds. Self-substrate inhibition was found at TCC concentration of 30 mg/L. The kinetics of TCC removal by entrapped and free cells fitted well with Edwards model. Scanning and transmission electron microscopic observations revealed that entrapment matrices reduced TCC-microbe contact, which lessened TCC inhibition. A live/dead cell assay also confirmed reduced microbial cell damage in the entrapped cell system compared to the free cell system. This study reveals the potential of entrapment technology to improve antibiotic removal from the environment.
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