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Long non-coding RNA Linc00320 inhibits glioma cell proliferation through restraining Wnt/β-catenin signaling

Shuaiwei Tian1, Wei Liu2, Yixin Pan2

  • 1Department of Pediatric Neurosurgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No.639 Zhizaoju Road, Shanghai 200011, China.

Biochemical and Biophysical Research Communications
|December 4, 2018
PubMed

Insights

This study identifies Linc00320 as a tumor-suppressive long noncoding RNA (lncRNA) in Glioma. Its decreased expression inhibits Glioma cell proliferation by targeting the Wnt/β-catenin signaling pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Numerous oncogenic long noncoding RNAs (lncRNAs) are implicated in Glioma progression.
  • The role of anti-oncogenic lncRNAs in Glioma remains largely unexplored.

Purpose of the Study:

  • To investigate the function of the HMGB1-regulated lncRNA, Linc00320, in Glioma.
  • To elucidate the mechanism by which Linc00320 affects Glioma cell proliferation and signaling pathways.

Main Methods:

  • Analysis of Linc00320 expression in Glioma tissues.
  • In vitro and in vivo studies to assess the effect of Linc00320 on Glioma cell proliferation.
  • Investigation of Linc00320's interaction with β-catenin and its impact on Wnt/β-catenin signaling.

Main Results:

  • Linc00320 expression is significantly decreased in Glioma tissues, correlating with poor prognosis.
  • Nuclear-localized Linc00320 inhibits Glioma cell proliferation both in vitro and in vivo.
  • Linc00320 binds to β-catenin, disrupting the β-catenin/TCF4 complex and inhibiting Wnt/β-catenin signaling.

Conclusions:

  • Linc00320 acts as a tumor suppressor in Glioma by inhibiting cell proliferation.
  • The HMGB1/Linc00320/β-catenin axis represents a novel regulatory pathway in Glioma progression.
  • Down-regulation of Linc00320 contributes to Glioma malignancy, suggesting its potential as a therapeutic target.
Keywords:
Cell proliferationGliomaHMGB1Linc00320Wnt/β-catenin signaling

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