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A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
Published on: September 18, 2013
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Development of migrating tendon-bone attachments involves replacement of progenitor populations
Neta Felsenthal1, Sarah Rubin1, Tomer Stern1
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Development (Cambridge, England)
|December 4, 2018
Summary
Researchers identified distinct progenitor cells that form entheses, crucial tendon-bone attachments. Sox9+ cells form stationary entheses, while Gli1 lineage cells form migrating entheses, offering insights for regenerative medicine.
Area of Science:
- Developmental biology
- Musculoskeletal system research
- Cell lineage tracing
Background:
- Entheses are vital tendon-bone attachment sites essential for musculoskeletal function.
- Entheses develop embryonically from Sox9+ progenitors and postnatally from Gli1 lineage cells.
- The transition from embryonic to mature enthesis and the relationship between Sox9+ and Gli1 lineages remain unclear.
Purpose of the Study:
- To investigate the transition from embryonic to mature enthesis development.
- To elucidate the relationship between Sox9+ progenitors and the Gli1 lineage in enthesis formation.
- To identify the onset and contribution of the Gli1 lineage to different entheses.
Main Methods:
- Utilized lineage tracing experiments in mice.
- Investigated the role of SHH and IHH signaling pathways in regulating Gli1 expression.
- Analyzed enthesis migration patterns during bone elongation.
Main Results:
- Identified the onset of Gli1 lineage contribution to various entheses.
- Demonstrated embryonic regulation of Gli1 by SHH signaling and postnatal maintenance by IHH signaling.
- Showed that Sox9+ cells differentiate into the Gli1 lineage in stationary entheses, while migrating entheses are populated by a distinct Gli1 lineage.
Conclusions:
- Enthesis development involves distinct progenitor populations: one for embryonic tissue establishment and another for maturation.
- Gli1 progenitors are established embryonically to form specific domains within the mature enthesis.
- Findings suggest different cell populations could be targeted for cell-based therapies for enthesis injuries.
Keywords:
EnthesisGli1Hedgehog signalingMouseMusculoskeletal developmentProgenitor cellR26R-ConfettiSox9More Related Videos
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