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Updated: Feb 1, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Exosomal MicroRNA Expression Profiling in Patients with Lung Adenocarcinoma-associated Malignant Pleural Effusion
Hiroyuki Tamiya1, Akihisa Mitani2, Akira Saito1
1The Department of Respiratory Medicine, The University of Tokyo Hospital, Tokyo, Japan.
Background/Aim:
Cytological analysis for diagnosing malignant pleural effusion associated with lung adenocarcinoma (Ad-MPE) shows limited sensitivity and novel diagnostic biomarkers are needed. The aim of this study was to evaluate the profile of four microRNAs (miRNAs) in exosomes in Ad-MPE and benign (non-neoplastic) pleural effusion (BPE).
Materials And Methods:
A total of 56 pleural effusion samples from patients with lung adenocarcinoma and benign diseases were collected. Exosomal miRNA expressions were evaluated using real-time quantitative reverse transcription polymerase chain reaction (RT-PCR).
Results:
The expression levels of miR-182 and miR-210 were significantly higher in the Ad-MPE than in the BPE samples. The receiver operating characteristics curve analyses of miR-182 and miR-210 for diagnosis of Ad-MPE yielded areas under the receiver operating characteristic curves of 0.87 and 0.81, respectively.
Conclusion:
These miRNA signatures may have a diagnostic potential for differentiating Ad-MPE from BPE.
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Clinical Manifestations:
MicroRNAs
MicroRNAs
Behavior of Gas Molecules: Molecular Diffusion, Mean Free Path, and Effusion
Lung Capacity

