Related Experiment Video
Updated: Feb 1, 2026

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)
Published on: June 27, 2025
Tumor-infiltrating Immune Cells in H&E-stained Sections of Colorectal Cancer Tissue as a Reasonable Immunological
Shinji Matsutani1, Masatsune Shibutani2, Kiyoshi Maeda1
1Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
Background:
The density of tumor-infiltrating lymphocytes has been reported to reflect the antitumor immune status, and many reports have shown that tumor-infiltrating CD8+ and total T-lymphocytes may be strong prognostic biomarkers in colorectal cancer. We previously reported that the density of tumor-infiltrating immune cells in hematoxylin and eosin (H&E)-stained sections may be an easily available prognostic biomarker. However, it remains unclear whether the density of tumor-infiltrating immune cells in H&E-stained sections accurately reflects the antitumor immune status.
Patients And Methods:
A total of 308 patients who underwent curative resection for stage II/III colorectal cancer were enrolled. The density of both tumor-infiltrating immune cells in H&E-stained sections and tumor-infiltrating lymphocyte subsets was assessed by immunohistochemistry.
Results:
The density of tumor-infiltrating immune cells in H&E-stained sections was significantly and positively correlated with that of tumor-infiltrating CD4+/CD8+/total T-lymphocytes.
Conclusion:
The density of tumor-infiltrating immune cells in H&E-stained sections may be a reasonable immunological biomarker.
More Related Videos
Related Concept Videos
Reason and Intuition
Reasoning
Inductive reasoning involves deriving generalizations from specific observations. This type of reasoning helps form beliefs about the world. For example,...
Cancer Stem Cells and Tumor Maintenance
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Deductive Reasoning
For example, a researcher can deduce specific predictions...
Inductive Reasoning
Inductive reasoning is common in descriptive science. A life scientist makes observations and records them. This data can be qualitative or...
Immunological Memory
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...

