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Updated: Feb 1, 2026

Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
Fluorescence assay to predict activity of the glycopeptide antibiotics
Vladimir Vimberg1, Radek Gazak2, Zsolt Szűcs3
1Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic. vladimir.vimberg@biomed.cas.cz.
Abstract:
Here, we describe a fluorescent assay developed to study competitive binding of the glycopeptide antibiotics to live bacteria cells. This assay demonstrated that the mechanism of action of the lipoglycopeptide antibiotics strongly depends on the hydrophobicity of the substitutes, with the best antibacterial activity of the glycopeptide antibiotics equally sharing properties of binding to D-Ala-D-Ala residues of the nascent peptidoglycan and to the membrane.
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