Reference values of nuchal fold thickness in an Iranian population sample

Considering that ethnicity and gestational age (GA) could affect the value of nuchal fold thickness (NFT) in mid-trimester, we aimed to determine the reference intervals of NFT values for each gestational week from 16 to 24 weeks of pregnancy among a group of Iranian pregnant women.

In this cross-sectional study, medical files of pregnant women who underwent fetal anomaly scanning at 16-24 weeks of gestation were reviewed and the following data were extracted: GA, value of NFT, value of nuchal translucency (NT) in their previous ultrasound study, if available, and head circumference (HC). The 5^th, 25^th, 50^th, 75^th, and 95^th percentiles of NFT for each gestational week were determined. The association between NFT and HC, GA, and NT were also determined.

Medical files of 882 pregnant women were studied. The expected 95^th percentile value of NFT between 16^th and 24^th weeks of gestation ranged from 4 mm to 5.9 mm. The mean (standard deviation) of NFT increased with GA from 2.67 (0.90) mm at 16^th weeks to 4.69 (0.71) mm at 24^th weeks. There was a significant positive association between NFT and GA (β = 1.11, p < 0.001), HC (β = 0.21, p < 0.001), and NT (β = 0.351, p < 0.001).

The findings of this study revealed that before the 20^th week of gestation, the appropriate cutoff value of NFT is 5 mm, and for 21^st to 24^th weeks, the proper cutoff is 6 mm. However, for providing more conclusive results, further studies with larger sample size and considering the impact of other influencing variables are recommended.