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Updated: Feb 1, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Basic Antibacterial Assay to Screen for Bacteriocinogenic Lactic Acid Bacteria and to Elementarily Characterize Their
1Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan. karakawa@okayama-u.ac.jp.
Abstract:
Bacteriocins ribosomally produced by lactic acid bacteria are antibacterial peptides expected to be used as a safe biopreservative and a fermentation controller in food industry. The modified agar-well diffusion method is most frequently used for antibacterial activity assay to screen for potentially bacteriocin-producing strains and to elementarily characterize their bacteriocins and the relatives. Here, I describe procedure of the modified agar-well diffusion assay in the details.
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